We wanted to know what kind of tests were conducted on Tian Xian Liquid and whether the liquid is the main product of the entire Tian Xian product line and we got the following results:

It seems that the Liquid is main product of the entire Tian Xian product line. We could not find any testing on Tian Xian capsules, plaster and others.
As far as Tian Xian Liquid is concerned, however we could find some convincing testing and laboratory experiments:

Tian Xian Liquid has obtained the following certificates:

- it has met the US Food and Drug Administration's (FDA) standard as a dietary supplement in December 1999.
- in December 2000 it has obtained a certificate listing it as a therpeutic good from the Australia Therapeutic Goods Administration (TGA).

Title: Immunomodulating Effects of Tian Xian Liquid on Peripheral Blood Mononuclear Cells and T-Lymphocytes from Patients with Recurrent Aphthous Ulcerations Source:
The American Journal of Chinese Medicine, Vol. 32, No. 2 (2004) 221-234 Copyright:World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine Author(s): Andy Sun Institute of Microbiology, College of Medicine, National Taiwan University, ROC Jean-San Chia Institute of Microbiology, College of Medicine, National Taiwan University, ROC Won-Bo Wang Institute of Microbiology, College of Medicine, National Taiwan University, ROC

Abstract: Recurrent aphthous ulcerations (RAU) represent a common oral mucosal disease with altered humoral and cellular immunities. In our institution, an immunomodulating agent, levamisole, is used to treat RAU with satisfactory therapeutic effect. Tian Xian Liquid is an extract of Chinese medicinal herbs with immunomodulating effects. To test whether Tian Xian Liquid has immunomodulating effects on antigen-stimulated proliferation response (PR) of peripheral blood mononuclear cells (PBMC) and T-cells isolated from RAU patients and to test whether Tian Xian Liquid is a potential drug for treating RAU, PBMC or T-cells isolated from RAU patients were incubated with lipopolysaccharides (LPS) from Escherica coli, phytohemagglutinin (PHA), staphylococcal enterotoxin B (SEB), glutaraldehyde-inactivated tetanus toxoid (TT), glucosyltransferase D (GtfD), or antigens of Streptococcus mutans in the presence or absence of Tian Xian Liquid. We found that Tian Xian Liquid significantly increased the LPS-stimulated PR of PBMC from active RAU patients, the GtfD-stimulated PR of PBMC and of T-cells from inactive RAU patients, and the S. mutans-stimulated PR of PBMC from inactive RAU patients. However, Tian Xian Liquid could also significantly reduce the SEB-stimulated PR of PBMC and of T-cells from active RAU patients and S. mutans-stimulated PR of T-cells from active RAU patients. These results suggest that Tian Xian Liquid can modulate the antigen-stimulated PR of PBMC and T-cells from RAU patients.
Therefore, it may be a potential immunoceutical agent for treatment of RAU.

Tian Xian Liquid Research Study Published in American Journal of Chinese Medicine, vol. 32 No. 2

Published Medical Research Tian Xian Research Study Published in American Journal of Chinese Medicine Title: Immunomodulating Effects of Tian Xian Liquid on Peripheral Blood Mononuclear Cells and T-Lymphocytes from Patients with Recurrent Aphthous Ulcerations Source: The American Journal of Chinese Medicine, Vol. 32, No. 2 (2004) 221-234 Copyright: World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine Author(s): Andy Sun Institute of Microbiology, College of Medicine, National Taiwan University, ROC Jean-San Chia Institute of Microbiology, College of Medicine, National Taiwan University, ROC Won-Bo Wang Institute of Microbiology, College of Medicine, National Taiwan University, ROC Abstract: Abstract: Recurrent aphthous ulcerations (RAU) represent a common oral mucosal disease with altered humoral and cellular immunities. In our institution, an immunomodulating agent, levamisole, is used to treat RAU with satisfactory therapeutic effect. Tian Xian Liquid is an extract of Chinese medicinal herbs with immunomodulating effects. To test whether Tian Xian Liquid has immunomodulating effects on antigen-stimulated proliferation response (PR) of peripheral blood mononuclear cells (PBMC) and T-cells isolated from RAU patients and to test whether Tian Xian Liquid is a potential drug for treating RAU, PBMC or
T-cells isolated from RAU patients were incubated with lipopolysaccharides (LPS) from Escherica coli, phytohemagglutinin (PHA), staphylococcal enterotoxin B (SEB), glutaraldehyde-inactivated tetanus toxoid (TT), glucosyltransferase D (GtfD), or antigens of Streptococcus mutans in the presence or absence of Tian Xian Liquid. We found that Tian Xian Liquid significantly increased the LPS-stimulated PR of PBMC from active RAU patients, the GtfD-stimulated PR of PBMC and of T-cells from inactive RAU patients, and the S. mutans-stimulated PR of PBMC from inactive RAU patients. However, Tian Xian Liquid could also significantly reduce the SEB-stimulated PR of PBMC and of T-cells from active RAU patients and S. mutans-stimulated PR of T-cells from active RAU patients. These results suggest that Tian Xian Liquid can modulate the antigen-stimulated PR of PBMC and T-cells from RAU patients. Therefore, it may be a potential immunoceutical agent for treatment of RAU.

Immunization Function Tests
Test conducted on laboratory mice concluded that Tian Xian Liquid:

1. Increases the phagocytosis of macrophage

2. Strengthens lymphatic conversion for the spleen

3. Raises the serum hemolysis reaction and anti-host reaction indicative of significant benefits toward immunization.

Removal of Free Radicals
Product Search
Clinical Research Studies on Tian Xian
To better understand the manner in which Tian Xian Liquid works, China-Japan Feida Union Co., LTD. facilitated an independent study by the Taipei FRC Biology Study Center. The study encompassed fifteen experimental topics and verification processes whereTian Xian Liquid was identified as subject FRC001 for the purpose of maintaining a completely unbiased and objective analysis of the product. The testing was concluded after two years where four primary directives were addressed.

The following is a summary of those results:
Experiments On The Removal Of Free Radicals
It is able to effectively remove different free radicals in the body.
It can remove super oxide free radical with a clearance capacity of 300,000 units of SOD activity per cc. It can remove free radical generated by white blood cells, which Vitamin E fails to effectuate.
It can remove hydroxyl free radical, which Vitamin E fails to effectuate.
It has the capacity to remove lipid peroxides more effectively than
Vitamin E.

Inhibitory Effect On Tumors

1. It has been demonstrated that Tian Xian Liquid inhibitory effects were not inferior to that of 5-Fu (chemical) when evaluated on laboratory mice affected with sarcoma and hepatocarcinoma and further, exhibited a quantum reaction, i.e. the larger the dose, the better the inhibitory effect which was greatly superior when compared to the reference group consisting of glossy ganoderma and green algaes.

Toxicology Experiments
In clinical studies,Tian Xian Liquid was able to pass very stringent
testing which included:
Acute Toxicity Test
Micro Nuclear Bone Marrow Cell Test
Sperm Distortion Tests
Ames Test

Animal Experiments:
Animal experiments have been conducted by (1) Free Radical Biology & Development Center, Taipei, in 1996; (2) Ching-Hsing Medical Foundation from 1999 to 2004; and (3) Tung General Hospital for clinical trial on T-cells from 2003 to 2004.

Type of Experiments conducted and place:
- Experiments on removal of free radical by Institute of Biophysics, Academia Sinica, Beijing Bradford Research Institute, CA, USA.
- Toxicology experiments by Institute of Biophysics, Academia Sinica, Beijing Bradford Research Institute, CA, USA.
- Immunization function tests by Institute of Biophysics, Academia
Sinica, Beijing Bradford Research Institute, CA, USA.
- Inhibition effect on tumor by Guangzhou Tumor Institute.

Eperiment Results:
Tian Xian Liquid is able to effectively remove:

- Superoxide free radical with clearance capacity of 300,000 unit of SOD activity per c.c.
- Free radical generated by white blood cells which vitamin E fails to effectuate.
- Hydroxyl free radical which vitamin C fails to effectuate.
- The capacity to remove lipide peroxide is much higher than that of vitamin E.

Experiments on Toxicology:
From animal experiments on mice, Tian Xian Liquid was able to pass stringent acute toxicity test, micro-nuclear of bone marrow cell test, sperm distortion test and Ames test respectively.

Experiments on Immunization Function:
Animal experiments on mice showed thatTian Xian Liquid will increase the phagocytosis of macrophage, strengthen lymphatic conversion for the spleen and raise the serum hemolysis reaction and anti-host reaction which indicates significant benefits to immunization.

Experiment on the Inhibition Effect on Tumors:
As the evidence of the test on mice in sarcoma and hepatocele, inhibition effect showed that Tian Xian Liquid is not inferior to chemical (5-Fu). Quantum effect reaction was observed which indicated that the larger the dosage, the better the inhibition effects. This was greatly superior to a reference group of glossy ganoderma and green algaes etc.

Conclusion of the animal experiments on Tian Xian Liquid conducted by Ching-Hsing Medical Foundation:
It was discovered that Tian Xian Liquid could modulate the antigen-stimulated PR of PBMC and T cells from RAU (Recurrent aphthous ulcerations) patients. Studies showed that Tian Xian Liquid also has anti-inflammatory and immunomodulating effects that may correct the altered cellular and humoral immunities in RAU patients. Therefore, Tian Xian Liquid can be concluded as a potential immunoceutical agent for RAU patients.

Conclusion on the animal experiments for the killing of K562 and U-937 cells?
Addition of Tian Xian Liquid at the dilution of 1/100 to complete PBMC or CD8+ cells from either RAU or EOLP patients significantly augmented the amount of killing of K562 cells. CD8+ cells, but not the CD4+ cells, are involved in the Tian Xian Liquid-stimulated killing of K562 cells by PBMC from RAU patients. In contrast, neither CD4+ nor CD8+ cells are involved in the Tian Xian Liquid stimulated killing of K562 cells by PBMC from EOLP patients. Tian Xian Liquid contains ingredients that can either activate NK cells to kill K562 cells or directly induce the lysis or apoptosis of the K562 and U937 cells.